Evaluation of the micronucleus and comet assays in patients with hepatitis B in Samarra city/Iraq

Document Type : Original Article

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Department of Pathological Analysis, College of Applied Sciences , University of Samarra, Samarra, Iraq

Abstract

Background: Hepatitis B virus (HBV) remains a significant global health concern, affecting millions of individuals worldwide.
Objective: In this study, we aimed to explore these assays, their relevance in evaluating genetic damage in patients with HBV, and their potential implications for public health in the region. 
Methods and patients: We employed the comet assay and MNi scoring to assess the induction of host DNA in the oral and renal epithelial cells of HBV-infected patients in comparison to healthy controls. The samples were collected from individuals (45 women and 51 men). Expert medical professionals have clinically determined that fifty healthy controls and forty-six HBV samples exhibit symptoms. They are between 15 and 80 years old.
Results: The results revealed that the highest mean of mn was in HBV patients (4.11) compared to the healthy control group (0.26). The results showed that the BN in the HBV patient group was lower (0.38) than in the healthy control group (3.61). Furthermore, the KL was higher in the health controls (1.83) than in the patients' group (1.78). Moreover, the TCWD was higher among the HBV and healthy control groups. Furthermore, there was no significant difference between the groups under study and healthy control groups at (p≤ 0.05).
Conclusion: The results of this study revealed that HBV is a serious cause of liver disease in Samarra.

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