Detection of AMPK Gene Expression in Mice and Its Relationship with Clopidogrel Effect, With the Study of Some Vital Variables

Document Type : Original Article

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Department of Pathological Analysis, College of Applied Sciences, University of Samarra, Samarra, Iraq.

Abstract

Background:
Plavix contains clopidogrel and belongs to a group of medicines called antiplatelet medicinal products. Platelets are very small structures in the blood that clump together during blood clotting. By preventing this clumping, antiplatelet medicinal products reduce the chances of blood clots forming (a process called thrombosis). adenosine monophosphate (AMPK)-activated protein kinase is a widely recognized common mechanism responsible for the treatment of Metabolic dysfunction-associated steatotic liver disease (MASLD)
Objective:
We hypothesized that clopidogrel may activate the AMPK pathway.
Methods: 30 male mice (MUS MUSCULUS) were taken. No statistically significant changes were observable in animal weights in all groups before and after the experiment, but the liver weight decreased with clopidogrel. by using Quantitative Real-Time PCR (qRT-PCR)
Results:
The AMPK gene expression was significantly higher in mice treated with clopidogrel than in controls.  Micronuclei arise primarily because of genetic damage to living cells. The micronucleus (MN), a nuclear body that can easily be seen in cells, is created when chromosomes break or segregate during cell division. The test was performed according to the Schmid (1975) method. This study intended to investigate whether Indian Plavix increases or decreases MN in mice.  PCEs *79.000 ± 4.109, MNiPCEs *11.400 ± 2.458, NCE *21.400 ± 3.747, and MNiRBCs *5.200 ± 1.686 significantly increases than in control. 
Conclusion:
We suggest that clopidogrel could ameliorate fatty liver in mice through enhancing phosphorylation of the AMPK pathway.  The results indicated that Indian Plavix increases MN in mice.

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